根尖牙乳头干细胞外泌体对牙髓干细胞增殖和分化的影响
陈跃敏

福建医科大学附属口腔医院，福建福州，350001；

摘要：目的:探讨根尖牙乳头干细胞外泌体（SCAP-Exo）对牙髓干细胞（DPSCs）生物学行为的调控，重点探讨其对细胞增殖及成牙本质/成骨双向分化能力的影响。方法:基于改良酶消化法（I型胶原酶/Dispase酶消化体系）分离培养根尖牙乳头干细胞（SCAP）和DPSCs，超速离心法提取SCAP-Exo，并通过透射电镜及WB验证其特性。采用CCK-8法、茜素红S染色及RT-PCR检测SCAP-Exo对DPSCs增殖、矿化能力及成牙本质分化相关基因（DSPP）的影响。结果:SCAP-Exo呈典型双层膜囊泡结构，表达外泌体标志蛋白Alix和CD9；SCAP-Exo（50/100 μg/mL）对DPSCs增殖无显著影响（P>0.05），但显著提升矿化结节形成量（P<0.0001）并上调DSPP基因表达（P<0.0001），而对成骨相关基因OCN、Runx2无显著调控作用。结论:SCAP-Exo可通过其携带的根尖牙乳头组织的特异性生物学信号促进DPSCs成牙本质向分化，为SCAP-Exo作为一种生物制剂应用于再生性牙髓治疗提供依据。

关键词：根尖牙乳头干细胞；牙髓干细胞；外泌体；成牙本质分化；再生性牙髓治疗

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