基于内切酶信号放大的双磁珠法与等离子体质谱联用测定循环肿瘤细胞
殷锦柠 彭泳岚 陈晓燕 陈祥卓 李潇婷（通讯作者） 肖博（通讯作者） 李彬（通讯作者）

深圳职业技术大学，广东深圳，518000；

摘要：为解决癌症早期筛查创伤大、早期检出率低及循环肿瘤细胞（CTCs）丰度极低难以检测的难题，本研究构建基于内切酶信号放大的双磁珠法与电感耦合等离子体质谱（ICP-MS）联用检测新方法。以MCF‑7细胞为靶标，磁珠MBs1偶联适配体并以DNA1封闭，识别靶细胞后释放DNA1；DNA1与磁珠MBs2上 DNA2‑Au NP互补配对，经Nb.BbvCI内切酶剪切循环释放大量金纳米粒子，通过ICP‑MS检测¹⁹⁷Au信号实现定量。该方法检出限20个/mL，线性范围 50~40000个/mL，R²=0.9973，对MCF‑7细胞特异性良好，操作简便、灵敏无创，为癌症早期筛查提供新型技术支撑。

关键词：循环肿瘤细胞；双磁珠法；内切酶信号放大；电感耦合等离子体质谱；金纳米粒子

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